AB1049 THE STATUS OF BREG CELLS IN PATIENTS WITH ANKYLOSING SPONDYLITIS

Background Ankylosing spondylitis (AS) is a common chronic inflammatory autoimmune disease that primarily affects the sacroiliac joints, spine and peripheral attachments of ligaments. Regulatory B cells (Bregs) are unique subpopulations with immune-regulating properties. Interestingly, different subsets Breg have distinct markers phenotypes participate in self-immune regulation ways. However, level AS remains debated. Objectives This study aims to clarify proportions unique, controversial cellular patients. Methods We explored proportion patients by searching literature through January 10, 2023, from 11 Databases. Random effects model was used pool data. Heterogeneity risk bias were examined I-squared index (I 2 ) statistic. Inconsistency evaluated using I Egger tests evaluate potential publication (STATA v.12.0). Results A total 7 case-control studies involving 278 237 healthy controls (HCs) included this study(Table 1). No significant difference evident between HCs[SMD=-0.324, 95%CI(-0.971, 0.323), P=0.327]. Because statistical heterogeneity observed [p<0.1, =89.2%], we conducted sub-analyses based on individual definitions cells. found CD19 + CD24 hi CD38 no HCs [SMD=-0.546, 95%CI (-1.126, 0.034), P=0.065](Figure The CD27 increased compared HCs[SMD=0.965, (0.339, 1.590), P=0.003] (t=1.35, p=0.236). Conclusion levels significantly patients, suggesting abnormalities cell numbers function critical causes development AS. Table 1. Characteristics meta-analysis. Author Publish year Case number cells’ definition ratio (mean/ median±standard deviation) PBMC/CD19 HC Yu Zhang 2022 42 30 AS:6.125±4.219 HC:9.021±4.527 PBMC Guo-Hui Xue 2015 38 AS:2.1±0.66 HC:3.44±0.8 Xiao-Wan Huang 2014 10 18 AS:7.15±2.0277 HC:6.87748±1.9131 Mingcan Yang 2020 67 50 AS:1.84812±2.1178 HC:2.03012±4.2419 Maria-Belen Bautista-Caro1 2017 AS:3.412±1.011 HC:4.361±1.744 Zhangyu AS:6.2±3.2 HC:8.6±4.0 Zhukangxiang 2021 22 AS:16.5±3.5 HC:13.4±2.9 Chenmeng 2016 15 AS:9.8±1.1 HC:9±1.4 Figure (A) Forest plot subgroup meta-analysis HCs. Acknowledgements work supported National Natural Science Foundation China (No. 82001740). Disclosure Interests None Declared.